plasmid (Addgene inc)
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Addgene inc
plasmid
Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+sgrna/AAV-U6-sgRNA-hSyn-mCherry+(Plasmid+%2387916)/pm42906853-47-12-18
Average 93 stars, based on 19 article reviews
Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+sgrna/AAV-U6-sgRNA-hSyn-mCherry+(Plasmid+%2387916)/pm42906853-47-12-18
Average 93 stars, based on 19 article reviews
plasmid - by Bioz Stars,
2026-10
93/100 stars
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Cloning:Article Title: Optimizing CRISPR/Cas9 approaches in the polymorphic tunicate Ciona intestinalis. Article Snippet: Briefly, oligo pairs were phosphorylated and annealed using the T4 polynucleotide kinase (NEB, Cat#: M0201). .. The obtained reaction was used for cloning into the Article Title: Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum. Article Snippet: .. CRISPR/Cas9 plasmids were generated by adding a sgRNA targeting the gene of interest into the linear Cas9 plasmid that was amplified from pACT:Cas9-GFP, Plasmid Preparation:Article Title: Optimizing CRISPR/Cas9 approaches in the polymorphic tunicate Ciona intestinalis. Article Snippet: Briefly, oligo pairs were phosphorylated and annealed using the T4 polynucleotide kinase (NEB, Cat#: M0201). .. The obtained reaction was used for cloning into the Article Title: In vivo human T cell engineering with enveloped delivery vehicles. Article Snippet: .. In brief, VSVG-pseudotyped Cas9-EDVs were produced by seeding 3.5–4 million Lenti-X cells (Takara Bio) into 10 cm tissue culture dishes (Corning) and transfecting the next day with 1 μg pCMV-VSV-G (Addgene plasmid no. 8454), 6.7 μg Gag-Cas9 (Addgene plasmid no. 171060), 3.3 μg psPax2 (Addgene plasmid no. 12260) and 10 μg Article Title: Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum. Article Snippet: .. CRISPR/Cas9 plasmids were generated by adding a sgRNA targeting the gene of interest into the linear Cas9 plasmid that was amplified from pACT:Cas9-GFP, Article Title: Establishment of human expanded potential stem cell lines via preimplantation embryo cultivation and somatic cell reprogramming. Article Snippet: We previously reported the derivation of expanded potential stem cells (EPSCs) by modulating signaling pathways involved in preimplantation embryogenesis.. These cells exhibit expanded developmental potential into embryonic and extraembryonic lineages, and we have shown that human EPSCs (hEPSCs) possess trophoblast differentiation potency for generating human trophoblast stem cells.. Here we report protocols for deriving stable hEPSC lines directly from morula or early blastocyst stages of human preimplantation embryos (hEPSC-em) and by reprogramming human dermal fibroblasts (human induced EPSCs) using six exogenous factors, as an extension to our previous protocols on deriving porcine EPSCs from preimplantation embryos and by reprogramming somatic cells. Produced:Article Title: In vivo human T cell engineering with enveloped delivery vehicles. Article Snippet: .. In brief, VSVG-pseudotyped Cas9-EDVs were produced by seeding 3.5–4 million Lenti-X cells (Takara Bio) into 10 cm tissue culture dishes (Corning) and transfecting the next day with 1 μg pCMV-VSV-G (Addgene plasmid no. 8454), 6.7 μg Gag-Cas9 (Addgene plasmid no. 171060), 3.3 μg psPax2 (Addgene plasmid no. 12260) and 10 μg other:Article Title: Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivity Article Snippet: The genes encoding Article Title: Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivity Article Snippet: The genes encoding Article Title: Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced pain facilitation Article Snippet: To produce the AAV vector for the Cre-switch system, vectors containing the promoters encoding EF1α were generated from CRISPR:Article Title: Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum. Article Snippet: .. CRISPR/Cas9 plasmids were generated by adding a sgRNA targeting the gene of interest into the linear Cas9 plasmid that was amplified from pACT:Cas9-GFP, Generated:Article Title: Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum. Article Snippet: .. CRISPR/Cas9 plasmids were generated by adding a sgRNA targeting the gene of interest into the linear Cas9 plasmid that was amplified from pACT:Cas9-GFP, Article Title: Establishment of human expanded potential stem cell lines via preimplantation embryo cultivation and somatic cell reprogramming. Article Snippet: We previously reported the derivation of expanded potential stem cells (EPSCs) by modulating signaling pathways involved in preimplantation embryogenesis.. These cells exhibit expanded developmental potential into embryonic and extraembryonic lineages, and we have shown that human EPSCs (hEPSCs) possess trophoblast differentiation potency for generating human trophoblast stem cells.. Here we report protocols for deriving stable hEPSC lines directly from morula or early blastocyst stages of human preimplantation embryos (hEPSC-em) and by reprogramming human dermal fibroblasts (human induced EPSCs) using six exogenous factors, as an extension to our previous protocols on deriving porcine EPSCs from preimplantation embryos and by reprogramming somatic cells. Amplification:Article Title: Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum. Article Snippet: .. CRISPR/Cas9 plasmids were generated by adding a sgRNA targeting the gene of interest into the linear Cas9 plasmid that was amplified from pACT:Cas9-GFP, |
